Cytokinin dehydrogenase activity
WebMar 4, 2014 · We have developed a N 6-dimethylallyladenine (cytokinin) dehydrogenase-based microbiosensor for real-time determination of the family of hormones known as cytokinins.Cytokinin dehydrogenase from Zea mays (ZmCKX1) was immobilised concurrently with electrodeposition of a silica gel film on the surface of a Pt … WebThe size and activity of the shoot apical meristem is regulated by transcription factors and low molecular mass signals, including the plant hormone cytokinin. The cytokinin status of the meristem depends on different factors, including metabolic degradation of the hormone, which is catalyzed by cytokinin oxidase/dehydrogenase (CKX) enzymes.
Cytokinin dehydrogenase activity
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WebSep 16, 2015 · Cytokinin dehydrogenase activity was measured using a modified end-point method described earlier . The reaction mixture (total volume of 0.6 ml in 1.5 ml tube) consisted of 100 mM McIlvaine buffer, pH 5.0 or 7.5, electron acceptor, substrate (dissolved in dimethyl sulfoxide that reached the final concentration of 2.5% in the reaction mixture ... Webcytokinin oxidase activity in the presence of copper-imidazole complexes was first observed by Chatfield and Armstrong [9]. This enhancement was not affected by …
WebApr 9, 2024 · Because CKme- tabolism modifying adeninemoiety par-tially shared purinesalvage pathway, nucleobase CKs readilyconverted correspondingnucleotides nucleosides(31). metabolicflow plays importantrole activeCKs (31). con-text, reduc-tion CKXactivity Habataki,NIL-Gn1a, increasedproduction CKconjugates overallCK activity … WebJul 1, 2002 · Spectrophotometric methods for determining the activity of cytokinin oxidase/cytokinin dehydrogenase (EC 1.5.99.12) were developed and optimized. A sensitive end-point method based on a combination of the electron acceptor 2,6-dichlorophenolindophenol and Schiff base formation of the reaction product with 4 …
WebApr 11, 2024 · 1.Introduction. Zinc (Zn), as one of the essential trace elements, regulates the activity and conformational stability of various proteins and enzymes by acting as a catalyst and regulatory or structural cofactor (Coleman, 1998).In plants, approximately 2,400 proteins require activation by Zn 2+ (Sinclair and Krämer, 2012), such as Zn-finger DNA … WebJul 1, 2002 · Two novelSpectrophotometric methods for determining the activity of cytokinin oxidase/cytokinin dehydrogenase (EC 1.5.99.12) were developed and optimized and can be used for kinetic studies with purified enzyme and is entirely substrate independent. Spectrophotometric methods for determining the activity of cytokinin …
WebEven though, it has already been established since 1980s that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; the potential applications of manipulating these enzymes have mostly been speculated to have a high potential in the biotechnology industry and spreads to agriculture, horticulture and agroforestry ... tsthelpWebJun 1, 2024 · The overexpression of CycD3 is sufficient to induce cytokinin-independent shoot formation in the calli 98, and the loss of CycD3 activity reduces the ability of exogenous cytokinins to induce ... ts theresaWebOct 31, 2015 · Cytokinins are hormones that regulate plant development and their environmental responses. Their levels are mainly controlled by the cytokinin oxidase/dehydrogenase (CKO), which oxidatively cleaves cytokinins using redox-active electron acceptors. CKO belongs to the group of flavoproteins with an 8α-N1-histidyl … ts the new tax lawWebMay 15, 2012 · Cytokinin dehydrogenase (CKX) catalyzes the irreversible degradation of cytokinins (CKs). CKs play a role in the regulation of hydrogen peroxide (H 2 O 2) accumulation while H 2 O 2 is involved in chlorophyll degradation. Here, we elucidated how CKX differentially regulates representative CK levels and indirectly influence H 2 O 2 … phlebotomy jobs near mcdonough gaWebSep 28, 2007 · The biological activity of cytokinin nucleotides is still unclear, since they usually show no activity in bioassays but can bind to certain cytokinin receptors … phlebotomy jobs near me brunswick gaWebSep 1, 2007 · Variable distribution of cytokinin oxidase/dehydrogenase activity (CKX: EC 1.5.99.12) in leaves of young pea seedlings (cv. Manuela) during the period needed for full leaf bud expansion was observed. phlebotomy jobs new orleansWebSep 28, 2007 · The biological activity of cytokinin nucleotides is still unclear, since they usually show no activity in bioassays but can bind to certain cytokinin receptors (Spichal et al., 2004). The adenine moiety of cytokinins can also be glycosylated, resulting in the formation of either N 7 - or N 9 -glucosides, which are much less active than the ... phlebotomy jobs near me with no experience