WebPeriodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly employed for the purification of antibodies in the biopharmaceutical industry [1] as well as in research and development. When purifying antibodies, Protein A is used as affinity matrix. WebMay 15, 2024 · The fruits of Sorbus pohuashanensis Hedl. (S. pohuashanensis) are rich in polyphenols with many beneficial effects such as anti-inflammatory, anti-tussive, anti-asthmatic and anti-cancer.In this study, five polyphenols, including three phenolic acids and two flavonoids, were successfully prepared from the fruits of S. pohuashanensis by high …
Continuous protein refolding and purification by two …
WebHIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY What every analytical chemist needs to know about this important new technique High-Speed Countercurrent Chromatography is the first book to be devoted entirely to this popular and fast-developing technique for separation and purification. It covers areas of particular interest to chemists … WebPartition chromatography theory and practice was introduced through the work and publications of Archer Martin and Richard Laurence Millington Synge during the 1940s. They would later receive the 1952 Nobel Prize in Chemistry "for their invention of partition chromatography".. Synopsis. The process of separating mixtures of chemical … nothic stalker
Countercurrent Separations - Chemistry LibreTexts
WebJan 7, 2024 · High-speed counter-current chromatography (HSCCC) was first developed in the early 1980s . It is a kind of chromatographic technology based on the theory of liquid-liquid distribution, and has been widely used in the isolation of natural products for its advantages of high recovery, simple sample preparation, high efficiency as well as ideal ... WebIn this work, a modified operational scheme is developed for continuous MAR of L-asparaginase inclusion bodies (IBs) using SEC-based periodic counter-current chromatography (PCC). The volumetric productivity of the modified SEC-PCC process is 6.8-fold higher than the batch SEC process. WebDec 10, 2024 · HUFA were purified by high-speed counter-current chromatography (HSCCC), characterized by LC-MS and MS/MS, and their antifungal activities were evaluated with 15 indicator fungal strains. The HUFA had different antifungal spectra when compared with unsaturated fatty acids with comparable structures but without hydroxy … nothic tactics